Researchers at the National University of Singapore (NUS) have developed a scalable, non-viral technology that efficiently ...

MicroRNA-27a has been shown to stimulate both the wingless-type integration site family, or Wnt signaling, and the bone morphogenetic protein pathways to actively promote bone regeneration, according ...

HCC1428, SKBR3, MCF7, and Jurkat cells were maintained at 37°C with 5% CO 2 RPMI1640 ... cells were treated with C48 at the indicated concentrations 1 day after transfection for a total of 5 days. In ...

Overexpression was confirmed using flow cytometry. Jurkat cells were nucleofected with 2–3 µg/ml of Piezo1-GFP construct. Overexpression was confirmed using flow cytometry. Transfected cells were ...

Lenti-HUWE1 and the corresponding control group Lenti were transfected into Jurkat cells for 48 h and then the cells were treated with 50 mmol/l CHX (MedChemExpress) for 0, 1, 2, or 4 h. The Ets-1 ...

In the past two decades, however, discoveries about the crucial role played by glia — a set of nervous system cells once thought to be mere supports for neurons — have rewritten chronic pain ...

Transmembrane TNF-expressing Jurkat cells were established by a stable transfection of human pro-TNF-α resistant to TACE-mediated cleavages as described previously (25). Cells were maintained in ...

Generation of stable Jurkat T cell lines HEK293T cells were co‐transfected with the pHR transfer plasmids with second‐generation packaging plasmids pMD2.G and psPAX2 (Addgene plasmid #12259 and #12260 ...

After 24 h, cells were analyzed for β-galactosidase activity by using a fluorogenic substrate-based assay. Plasmid DNA transfection into HeLa, IMCD, 3T3-L, PC12, and Jurkat cells by Lipofectamine 2000 ...

are efficient transfection agents in clinically relevant and difficult-to-transfect human cells (Jurkat T cells, primary T lymphocytes) and (ii) can efficiently deliver siRNA to human primary T ...