eLife24d
Sir2 and Fun30 regulate ribosomal DNA replication timing via Mcm helicase positioning and nucleosome occupancy
Cells carrying Mcm2-Mnase were subjected to ChEC as described above, except that the SDS was omitted from the stop buffer to allow subsequent spheroplasting. DNA was extracted from spheroplasts using ...
eLife24d
Sir2 and Fun30 regulate ribosomal DNA replication timing via MCM helicase positioning and nucleosome occupancy
qRT-PCR analysis of c-pro transcript and rDNA copy number RNA was extracted from logarithmically growing cells after spheroplasting using an RNA-Easy column. The sequences of the primers used in ...
Nature10d
Beyond toothpicks: new methods for isolating mutant bacteria
New single-cell screening technologies have revolutionized research into the genetics, evolution and biotechnological applications of bacteria. This Review focuses on the uses of autofluorescent ...