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Somatic cell hybridization is widely used to study the control of gene regulation and the stability of differentiated states. In contrast, the application of this method to germ cells has been limited ...
Total RNA was isolated from cells using RNeasy kit with DNase treatment (Qiagen). RNA probes for microarray hybridization were prepared and hybridized to Affymetrix HG U133 plus 2 oligonucleotide ...
These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization ...
The May 20, 2008, article by Sequist et al entitled, “First-Line Gefitinib in Patients With Advanced Non-Small-Cell Lung Cancer Harboring Somatic EGFR Mutations” (J Clin Oncol 26:2442–2449, 2008) was ...
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