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Frontiers4mon
Identification and verification of the optimal feature genes of ferroptosis in thyroid-associated orbitopathy
Orbital adipose tissue was lysed with RIPA buffer (Solarbio, China) to extract protein, which was transferred to PVDF membrane by SDS-PAGE gel electrophoresis. PVDF membrane was immersed in 5% skim ...
Nature9mon
Imaging FRET between spectrally similar GFP molecules in single cells
The individual cleavage products were resolved on denaturing, nonreducing SDS–PAGE gels. Measurements in solution were performed on 20 μl droplets spotted on MatTek coverslip dishes (MatTek ...
Frontiers3y
Aerially Applied Zinc Oxide Nanoparticle Affects Reproductive Components and Seed Quality in Fully Grown Bean Plants (Phaseolus vulgaris L.)
In addition, the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pattern of bean seed proteins was performed using the extracted total protein. Briefly, the seeds were grounded ...