but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton Smith and Kent Wilcox from Johns Hopkins University discovered “endonuclease R” (later named HindII) ...

For example, Sanger sequencing currently costs ∼$4 ... multiplexing of long-read sequencing results of multiplexed samples. During plasmid construction, a restriction enzyme digestion test can ...

Recombinase-based methods allow scientists to move pieces of DNA from plasmid to plasmid without restriction enzymes, simplifying and expediting the cloning process. It also prevents the aggravation ...

The PCR products and p3xFLAG-CMV™-14 were digested using EcoR I and Xba I restriction enzymes and ligated using T4 DNA ligase ... with 2 μg p3xFLAG or Bid-FLAG plasmid. At 24 h post-transfection, the ...

The bar chart shows the predicted functions of the 221 composite ATPase proteins are nucleic acid and amino acid interacting enzymes. For each category, a representative example of a composite ATPase ...

Depletion of FMRP elevated viral translation and enhanced ZIKV infection, indicating that FMRP is a ZIKV restriction factor. We further observed that an attenuated ZIKV strain compromised for sfRNA ...