The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA–DNA base-pairing to target foreign DNA in bacteria.

Researchers have engineered a compact, high-specificity IscB variant, NovaIscB, for safe, persistent in vivo epigenome ...

coli EndoV is equally active on both single- and double-stranded RNA. These are the novel findings implying a role for endonuclease V in RNA metabolism. Figure 1: Various incision activities for E.

The yeast nuclear endonuclease Rnt1 has a well-characterized role in the maturation of many ncRNA precursors. However, the scope and consequence of its function in mRNA degradation pathways is much ...

CRISPR–Cas13—a class 2 type VI RNA endonuclease with two higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domains for RNA cleavage—targets and degrades RNA. The RNA targeting has ...

The research is published in Nature, in the paper, “Fanzor is a eukaryotic programmable RNA-guided endonuclease.” People have long wondered whether systems similar to the well-known bacterial ...

Doudna is one of two corresponding authors of a paper in the journal Science describing this work titled "A programmable dual RNA-guided DNA endonuclease in adaptive bacterial immunity." ...

The review paper "CRISPR Beyond: Harnessing Compact RNA-Guided Endonucleases for Enhanced Genome Editing," authored by Feizuo Wang from National University of Singapore, presents a comprehensive ...

Respiratory syncytial virus (RSV), SARS-CoV2, influenza virus, poliovirus, and measles—all single-stranded RNA viruses—are ... stress granules from an RNA endonuclease RNase L that cleave ...

A guide RNA is designed to target the mutant Tmc1 allele and recruits Cas9 endonuclease to make a double-stranded cut. Random insertions and deletions are incorporated into the cut, and the mutant ...

RNA serves multiple functions in the body ... of the Fanzor proteins showed that they are DNA-cutting endonuclease enzymes that use nearby non-coding RNAs known as ωRNAs. These ωRNAs target ...