PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

RPA operates at a constant low temperature (37-45 °C) with only two specific primers, offering a rapid and sensitive alternative to PCR. In this webinar, we will demonstrate the use of RPA reaction as ...

--(BUSINESS WIRE)--Biotech and pharma labs performing endpoint polymerase chain reaction (PCR) can amplify samples ... primer-dimer and nonspecific DNA amplification. The master mix has been ...

NUCLEIC ACID AMPLIFICATION AND DETECTION METHODS developed in the past decade are useful for the diagnosis and management of a variety of infectious diseases. The most widely used of these methods is ...

Rapid PCR-based methods exist that take around 1 hour. CRISPR-Cascade, including sample preparation, requires about half an hour, making it the fastest available bloodstream infection diagnostic.

Methods: Our approach involves a two-step PCR amplification process. The initial round generates exon specific PCR products, which are then used as template for real-time PCR mutation detection ...