Lipofection of nondividing cells is inefficient because much of the transfected DNA is retained in endosomes, and that which escapes to the cytoplasm enters the nucleus at low rates. To improve ...

⁴ Maximizing NK cells via traditional approaches such as electroporation, lipofection, or viral approaches is even more challenging than modifying T cells as NKs are considerably more resistant ...

We transfected plasmids pCDNA3–Tbx5, pCDNA3–G80R or pCDNA3–R237Q into P19CL6 cells using Tfx-50 lipofection reagents (Promega). We isolated at least three independent clones of each plasmid.

2009). Non-viral reprogramming methods vary and include transfection and electroporation, among others. The most common chemical method is lipofection–liposome-based transfection. By means of ...

Despite utilizing the high-performing PE4max system and optimized epegRNAs, the intended editing efficiency remains constrained with lipofection-based delivery of the prime editing machinery, ...

Emerging techniques for mapping mRNAs within the subcellular compartments of live cells hold great promise for advancing our understanding of the spatial distribution of transcripts and enabling the ...

† The John B. Pierce Laboratory, Inc., New Haven, Connecticut 06519, United States ‡ § ‡ Department of Cellular and Molecular Physiology and § Department of Neuroscience, Yale University School of ...