Initially, the enriched CD34+ HSCs were transduced with the clinical-grade lentiviral vector CD68-ET3-LV (Fig. S1) according to a double-exposure transduction protocol (Protocol P1 in the ...

Takara Bio’s dissolvable, microfluidic gene delivery enhancer transforms viral transduction to enable advances in cell and gene therapy research.

For example, spinoculation—the use of a centrifuge to bring lentivirus and cells in closer proximity—and chemical enhancers are commonly used techniques to increase transduction efficiency.

Takara Bio USA, Inc., a wholly owned subsidiary of Takara Bio Inc., announced the launch of the Lenti-X™ Transduction Sponge, a first-to-market dissolvable microfluidic transduction enhancer that ...

With the fast progress and deeper understanding of CAR-T cell therapy, finding a better solution other than lentivirus transduction is more requested, to overcome the functional limitation on random ...

The lentiviral construct that was used in this gene-insertion therapy included the natural human Artemis promoter to avoid toxic overexpression of Artemis 17,18 and yet yield sufficient ...

New assay measures lentiviral vector transduction efficiency in days AVROBIO to present data on the transduction assay at 4th Annual Gene Therapy Analytical Development Summit SOUTH SAN FRANCISCO, ...

We then integrated a single transduction step to this protocol, previously optimized using a lentivirus that engineers expression of eGFP to consistently obtain at least 40% eGFP‐expressing cells ...