Viral gene delivery is a ubiquitous laboratory technique with a myraid of uses. Understanding the basics of gene delivery workflows, from vector selection and cloning to transduction, is a fundamental ...

Lentiviral transduction is a popular and powerful gene delivery method for downstream applications such as cell line generation, therapeutic model generation and CAR-T research. However, current ...

But recent work to optimize non-viral editing efficiency is starting to change the picture, highlighting the advantages of non-viral CRISPR ... lentivirus.” One way to achieve non-viral ...

We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and gene knockout and knockin using CRISPR/Cas9 technologies. 1. STABLE CELL LINE GENERATION ...

This study “harnesses CRISPR pooled screening technology to help us figure out where the functional regions in the noncoding genome [are],” he explained. Gersbach and colleagues created lentiviral ...

Here we asked if increasing NaCl tonicity in the vector formulation enhances the transduction efficiency of 3 gene therapy vectors: adenovirus, AAV, and lentiviral vectors. We observed that vectors ...