Study: Re-formation of synaptic connectivity in dissociated human stem cell-derived retinal organoid cultures.Image Credit: Kateryna Kon/Shutterstock Background. Synapse-forming retinal cells ...

"We confirmed for the first time in a non-human primate model that embryonic stem-derived retinal organoid sheet transplantation facilitates the closure of macular holes," says senior study author ...

My team has developed a method to transplant retinal sheets into degenerating retinas. 1 Because the retina has a very complex structure, it would be very difficult to get the photoreceptors to ...

Using immunocytochemistry techniques, they characterized proteins associated with specific retinal cells present at ...

That process produces actual retinal neurons organized as complex tissue, without any external stimulation of development pathways during specialization. The method has its limitations, though.

Ludwig AL, et al. Re-formation of synaptic connectivity in dissociated human stem cell-derived retinal organoid cultures. PNAS. 2023;120(2):e2213418120. Beier KT. The serendipity of viral ...

a, Colour bar of the D-FFOCT images with a consistent colormap for (b,c). b, Image of a D29 retinal organoid, showing multiple cells with different dynamic profiles. c, Image of a D51 retinal ...

The researchers found that the modified rabies virus traversed across the synapse to stain all three major retinal cell types 20 days after removal from the retinal organoid.

Using deep Hi-C sequencing, a tool used for studying 3D genome organization, the researchers created a high-resolution map of chromatin in a human retinal organoid at five key points in development.

Researchers in Germany now have another efficient way to make 3-D retina organoids, which mimic the organ's tissue organization, from mouse or human stem cells.