Due to the sudden rupture of the aortic wall, aortic dissection is a life-threatening condition that requires immediate ...

Researchers at the Max Planck Institute for Evolutionary Anthropology have addressed a longstanding challenge in CRISPR ...

Genetic defects are exceedingly common, which is not surprising considering just how many cells make up our bodies, including our reproductive cells. While most of these defects have no or only ...

Duke University Medical Center-led research has identified a human-specific DNA enhancer that regulates neural progenitor ...

TM6SF2 GKO mice fed an HFD showed elevated liver and intestinal lipid deposition compared to CON mice. The gut microbiota of HFD-fed TM6SF2 GKO mice exhibited a decreased Firmicutes/Bacteroidetes ...

8 This led to the development of programmable nucleases that can create site-specific DNA DSBs ... In germline editing, KO (knockout) or KI (knockin) mice are generated by injecting CRISPR-Cas9 ...

These indels are of varying size and, when targeted within protein coding sequences, can create inactivating ... considering that the liver specific-knockout model has a surprisingly moderate ...

A research group led by Wei Li at the Chinese Academy of Sciences has created mice born from two male parents ... not a small feat to make those 20-knockout stem cells while maintaining the ...

It’s a new way to create “bi-paternal” mice ... The team used gene editing to knock out imprinted genes altogether. Around 200 of a mouse’s genes are imprinted, but Li’s team focused ...

The authors indicate that sgRNA design changes in addition to changing the molar ratio of Cas9MRNA:sgRNA improved the ability to generate biallelic homozygous ... However, the use of FGF5 knockout ...