One major and costly bottleneck within the drug discovery process is the creation of the modified cells needed for HTS cell-based assays for screening ... protein (GFP) as a marker of transfection ...

EGFP was fluorescently quantified on a DeNovix QFX fluorometer using the BioVision GFP Quantitation Kit. A 200 μL total assay volume was prepared in 0.5 mL, thin-wall PCR tubes. To make 1 μg/μL ...

"TheGreenScreen HC and Ames II assays, when combined ... gene to the production of Green Fluorescent Protein (GFP). Cells that haveincurred DNA damage upon exposure to a test compound express ...

Image-based high-content screening assays, demand solutions for image segmentation and cellular compartment encoding to track critical events – for example those presented by GFPreporters within cell ...