In this study, we critically evaluate the nanobody epitope prediction performance of two leading models: AlphaFold3 and AlphaFold2-Multimer (v.2.3.2), highlighting their strengths and limitations. Our ...

For immunoprecipitation, the dimerized GFP nanobody construct LaG-16–G4S–LaG-2 (green lobster; gift from Michael Rout), mTOR 7C10, and Normal Rabbit IgG (2729S; Cell Signaling Technology, Danvers, MA) ...

2013), while the other one was recognizing green fluorescent protein (GFP) (Kubala et al., 2010). In order to find the best conditions for cell-free Nb synthesis with regard to protein yield and ...

Cells are surrounded by a membrane that separates the outside of the cell from its inside. Proteins called ion channels are embedded within this membrane and allow charged ions to move in and out of ...

Here, we have developed and used a novel, nanobody-based toolset, the GrabFP system, to interfere with the localization of GFP-tagged proteins along the apical-basal axis in the larval wing imaginal ...