To test this approach, we inserted a green fluorescent protein (GFP) nanobody (GFPnb) into the VP1 protein of AAV-DJ inside the capsid and replaced the AAV payload plasmid with a GFP expression ...

20 µL of this mixture was transferred to a 0.5 ml protein LoBind tube and mixed with 640 pmol of SPYtag-GFP nanobody and incubated at 4 °C for 10 h. The beads were washed twice and resuspended with 25 ...

Department of Chemistry, University of California, Berkeley, California 94720, United States Department of Chemistry, University of California, Berkeley, California 94720, United States Department of ...