Figure 1: Delivery of synthetic shRNA into Nanog-GFP mouse ES cells. M15 ES cell medium with leukemia inhibitory factor (LIF): DMEM Knockout (Gibco), 15% fetal bovine serum (FBS; StemCell ...

The sequence for the desired fluorescent label, GFP, YFP or mCherry, is inserted in the portion of the Chrna coding for the intracellular loop of the receptor subunit in question. All of these mice ...

Figure 1: Reconstitution of hematopoiesis with GFP-expressing peripheral-blood cell progeny in lethally irradiated mice after transplantation of GFP-transduced BM. Figure 2: Engraftment of BM ...

To understand this process better, Zernicka-Goetz set out to investigate if human embryonic development resembled that of mice. She and her team first tracked cell lineage from the two-cell stage; ...