EGFP was fluorescently quantified on a DeNovix QFX fluorometer using the BioVision GFP Quantitation Kit. A 200 μL total assay volume was prepared in 0.5 mL, thin-wall PCR tubes. To make 1 μg/μL ...

The assay, termed FPP, provides a fluorescence readout in response to trypsin-induced destruction of GFP attached to a protein of interest before and after plasma membrane permeabilization.