The yeast nuclear endonuclease Rnt1 has a well-characterized role in the maturation of many ncRNA precursors. However, the scope and consequence of its function in mRNA degradation pathways is much ...

Credit: Luisa P Oswalt/Shutterstock. Anheuser-Busch InBev has unveiled plans to invest $300m in its manufacturing operations across the US this year. In a statement, AB InBev, which owns brands ...

Endonuclease III (EndoIII), a key enzyme in the base excision ... This interpretation is consistent with previous structural studies that show protein binding to DNA via its active site, which ...

Inteins that contain an additional site-specific endonuclease domain display genetic mobility via a process termed “homing” and thereby act as selfish DNA elements. We elucidated the crystal ...

Facilitation of the eversion of a target nucleotide into the enzyme active site is mediated by some non–B-form regions ... In general, data obtained in the research on the specificity of AP ...

S1 and S2). The sequence alignment units demonstrate the highest degree of similarity in the region harboring the endonuclease active sites. A search with the sequence of the YEAST subunit sen54 ...

Using X-ray crystallography, we show how these inhibitors coordinate the two-metal endonuclease active site and engage the active site residues. Two structures also reveal an induced-fit mode of ...

If the active site of the endonuclease is blocked by an inhibitor the enzyme cannot bind its normal substrate, the host cell mRNA, and viral replication is prevented. The active site of the ...

We have engineered a variant of the I-AniI homing endonuclease that nicks its cognate target site. This variant contains a mutation of a basic residue essential for proton transfer and solvent ...

Figure 1: Hypothetical model of the cation-π sandwich of the eukaryotic tRNA-splicing endonuclease. Figure 3: Model of the archaeal and eukaryotic tRNA-splicing endonuclease. Yeast tRNA-splicing ...

In this report, crystallographic analysis shows that the global architecture of the bI3 maturase is unchanged from its DNA-binding homologs; in contrast, the endonuclease active site, dispensable ...