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Scanning electron microscopy revealed that untreated biofilms retained their dense, intact structure, while those exposed to the microrobots showed significant disruption and cell loss. Crystal violet ...
Biofilm abundance was quantified using crystal violet staining and spectrophotometry, and community composition was characterized through scanning electron microscopy (SEM) image analysis. Here, we ...
Crystal violet staining can be used for biofilm evaluation. A color meter is used to analyze different staining situations to determine the biofilm quantity. However, using a color meter for color ...
Biofilm in this plate was then visualized by crystal violet staining as described above. F. tularensis was grown for 7 days in a 24-well plate in CDM. The entire contents of the well was disrupted by ...
1 Centre of Biological Engineering (CEB), Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), University of Minho, Braga, Portugal 2 Division of Infectious Diseases, University of Alabama at ...
Biofilm formation by the indicated strains was displayed with crystal violet staining (up) and quantified with optical density measurement (down). SiaC is required for the function of SiaD. Biofilm ...
SiaB‐ and SiaC‐regulated biofilm formation is dependent on SiaD. Biofilm formation by the indicated strains was displayed with crystal violet staining (up) and quantified with optical density ...
photographs of 48-well plate biofilms after crystal violet staining and washing/drying; (xiii) photographs of 48-well plate biofilms after Congo Red staining and washing/drying; (xiv) crystal violet ...
One of the first staining assays used in biofilm analysis was the crystal violet assay. In this method, the negatively charged molecules within a biofilm (i.e. polysaccharides of the EPS and ...
Assessment of biofilm formation currently relies on a 96 well microtitre plate assay, which usually involves the colourimetric detection of stain (typically crystal violet) removed from previously ...