The resulting experimental mouse is hemizygous for Cre and homozygous for loxP (D). This is the necessary genotype required to conditionally knock out the target gene in the specific tissue. Laura is ...

The most straightforward way to examine the pattern of Cre-mediated recombination in the various tissues of the mouse is Southern blot analysis distinguishing wild-type, loxP-flanked and ...

Related Links: Jackson Laboratory / Mutant Mouse Regional Resource Center (MMRRC ... On binding tamoxifen, the complex is translocated to the nucleus where cre activates recombination of loxP-flanked ...

The cerebella from c-kit IRES-Cre mice injected with Cre-reporter virus at PND (pups shortly after birth) 0 or PND 64. Tissue was prepared from animals 27 and 14 days after injection ...

The Yong Xu lab is interested in novel neural circuits, neurotransmitters and intra-neuronal signals that are critical for coordinated control of metabolism and behavior. We generate unique mouse ...

In mice, chimaeras can also be generated by mixing embryonic cells of different genotypes. With the introduction of sequence-specific recombination systems such as FLP/FRT or Cre/LoxP, not only ...