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The Type II CRISPR-Cas9 system, derived from Streptococcus pyogenes (SpCas9), was the first to be characterized and widely applied in genome editing. Cas9, guided by a single-guide RNA (sgRNA)—a ...

Biosensors and Nanobiotechnology Laboratory, Integrated Science Building, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong BE1410, Brunei Darussalam ...

In this study, we demonstrated the exploitation of CRISPR/Cas9 as a high precision system for editing the composition and function of eECMs. Human mesenchymal stromal/stem cell (hMSC) lines were ...