Steps 1–14, construction of CRISPR-Cas9 vector for CRIS-PITCh (v2): 1 week Steps 15–25, construction of CRIS-PITCh (v2) donor vector: 3 d ...

Interestingly, nano-vectors loaded with Cas9 RNP can directly enter the nucleus to perform CRISPR/Cas9-mediated genome editing. A poly (d,l-lactide-co-glycolide) (PLGA)-based nano-vector for Cas9 ...

In addition, first-generation CRISPR systems (Cas9 and Cas12a) are too large for efficient in vivo delivery via a single adeno-associated viral (AAV) vector. Now, new preclinical research ...

Conventional CRISPR nucleases, including Cas9 and Cas12a, exceed the packaging limits of a single AAV vector, necessitating dual-AAV strategies that reduce efficiency. Smaller CRISPR systems such ...

In the latest episode of Touching Base, we discuss big news in CRISPR along with updates in the business side of biotech.

CRISPR-Cas9 identifies chosen sequences in DNA to produce precise cuts. Credit: Marius Walter / CC4.0 The vector used to deliver the CRISPR-Cas9 complex to cells was too bulky for use in a ...

while Intellia will bring its proprietary Nme2 CRISPR/Cas9 systems that are adapted for viral vector delivery and designed to precisely modify a target gene. Gene editing has so far been confined ...

Vector offers custom design and manufacturing services for AAV and adenovirus, as well as catalog vectors for overexpression, shRNA, miRNA, and CRISPR. Vector’s products have been referenced in ...

Adeno-associated viruses are the most common CRISPR vector, but they allow CRISPR components to persist in cells after editing is complete, leaving them vulnerable to off-target mutations.