and then tested several voltages to find one that delivers the CRISPR/Cas9 components inside the cell without causing it harm. In addition to faster throughput, the researchers' electroporation ...

Since electroporation can damage some cells, and other cells appear to resist it altogether, the strategy could provide a promising alternative for CRISPR delivery. The Feldan shuttle is inspired ...

This article offers strategies to optimize and improve CRISPR-Cas9 delivery even in hard-to ... An attractive alternative is electroporation-based Nucleofection™ technology.

Techniques such as electroporation have facilitated efficient ... thereby demonstrating the evolving landscape of CRISPR-Cas delivery methods and their potential to overcome current limitations.

Efficient and reliable delivery of DNA ... stem and progenitor cells (HSPCs) after CRISPR-Cas9 transfection utilizing the MaxCyte ® electroporation technology to address this issue.

The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation ... One challenge has been the efficient and consistent delivery of a range of gene-editing tools into human ...

(2) Researchers deliver CRISPR system components using different methods, including conjugation, transformation by heat shock or electroporation ... in it still have many challenges ahead. DNA ...

If the first CRISPR decade was marked by a mad dash to ... so far the second seems to be all about three words: “Delivery. Delivery. Delivery.” Gene-editing technologies hold huge promise ...

In the paper, characteristics of three forms of CRISPR/Cas9 cargos are outlined. Three delivery forms of the CRISPR/Cas9 system are plasmids, mRNA/sgRNA, and ribonucleoprotein (RNP) complexes ...