Industrial fermentation using bacteria, yeast and other microbes to produce specific products has the potential to reduce ...

the biotechs would combine ViaCyte’s stem cell expertise with CRISPR’s gene editing technologies to develop a potential beta-cell replacement product that could help patients produce their own insulin ...

Subsequently, DNA repair pathways like non-homologous end joining (NHEJ) or homology-directed repair bring the Cas9 and sgRNA together to ultimately modify the genome. The incorporation of CRISPR ...

Whilst genome editing with CRISPR-Cas9 is effective, it runs the risk of off-target effects and excessive mutations, which can lead to toxicity. To reduce the likelihood of these occurring, ...

On average, a bacterial genome contains around 30 biosynthetic gene clusters, potentially encoding 30 different natural products. 4 CRISPR/Cas9 gene engineering is expanding the range of these ...

A comprehensive study headed by researchers at Sanford Burnham Prebys has shown that gene editing—specifically gene knockout (KO)—using CRISPR-Cas9 technology can favor cells with mutated ...

This method, which is still being researched, uses the famous Nobel Prize winning gene-editing technology, CRISPR-Cas9. Scientists from the University of Sherbrooke in Canada used the technology ...

The PX330 vector (#42230, Addgene, United States), Cas9 expression plasmid with pX330-U6-Chimeric-BB-CBh-hSp-Cas9 construct, was linearized by BpiI at 37°C for 30 min. The product with sticky ...

By Katherine J. Wu A powerful gene-editing tool called Crispr-Cas9, which this month nabbed the Nobel Prize in Chemistry for two female scientists, can cause serious side effects in the cells of ...