On May 12, 2025, the U.S. Court of Appeals for the Federal Circuit issued a long-awaited decision in Regents of the University of California v.

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Here, authors leverage a split crRNA mode to construct spatially blocked split CRISPR-Cas12a system. This approach establishes a low-background, highly sensitive platform for small molecule detection.