AZoLifeSciences on MSN4d
CRISPR-Cas9 Off-Target Effects: Challenges and Solutions
The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...
NanoCas, a smaller version of CRISPR tested with a single AAV, delivers on-target results
Mammoth Biosciences researchers have developed NanoCas, an ultracompact CRISPR nuclease, demonstrating its ability to perform ...
Nature7d
CRISPR-Cas9 genome editing articles from across Nature Portfolio
CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...
gut.bmj14d
CRISPR/Cas9: at the cutting edge of hepatology
The sequence required immediately downstream of the target sequence. The PAM varies depending on the bacterial origin of the Cas9 protein. DSB: double-strand break. CRISPR/Cas9 introduces a blunt DSB ...
Frontiers16d
Imaging Unique DNA Sequences in Individual Cells Using a CRISPR-Cas9-Based, Split Luciferase Biosensor
An extensive arsenal of biosensing tools has been developed based on the clustered regularly interspaced short palindromic repeat (CRISPR) platform ... to sensitively detect unique copies of a target ...
Frontiers28d
A systematic screening assay identifies efficient small guide RNAs for CRISPR activation
The discovery of clustered regularly interspaced short palindromic repeats (CRISPR) DNA segments and CRISPR-associated proteins (Cas), known as CRISPR/Cas, has revolutionized biomedical research ...
CRISPR: From Discovery To Clinic—And Charting A Path Forward
No doubt 2025 will usher in many more advances in CRISPR therapies, thanks to the FDA’s new Platform Technology Designation ...
The EMBO Journal8d
A CRISPR-Cas9 screen reveals genetic determinants of the cellular response to decitabine
A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...