CRISPR-Nickase leverages the fact that humans have two copies of each gene—one from each parent. Cas9, the molecular scissors that excise target genes, has two active sites that severs each copy.

7 Besides protein engineering, another method to effectively reduce off-target effects is by employing CRISPR nickase to modify one nuclease domain in only one strand of DNA. 6 Unlike normal Cas9 ...

A team of researchers at Karolinska Institutet has developed a novel tool for genetic research. The study, published in ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into the roles that specific genes play in many diseases. But to date this ...

Over the past 15 years, developments in the gene-editing technique known as CRISPR-Cas9 have produced significant new ...

Human extracellular matrices can be edited in their composition using the CRISPR/Cas9 system, leading to materials exhibiting tailored regenerative capacities.

The Cas9 nickase cuts the unbound complementary strand ... “It’s not as simple a system as the original CRISPR-Cas9 or base editing where you have that single guide RNA and you target the site,” ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Among his recent work at Drexel is the development of technologies based on the CRISPR-Cas9 system for genome mapping and sequencing, which have broad applications in molecular diagnostics, cell/gene ...