followed by western blot analysis. The CRISPR/Cas9 system was utilized to generate Tet2 knockout cells. Lentiviruses were produced by co-transfecting HEK293T cells with plasmids containing sgRNA (8μg) ...

Join us for an insightful webinar on the latest advancements in cell and gene therapy, focusing on the innovative Cas9-CLIPT technology. Cas9-CLIPT (Cleaved, Linearized with Protein Template ...

In this study, we demonstrated the exploitation of CRISPR/Cas9 as a high precision system for editing the composition and function of eECMs. Human mesenchymal stromal/stem cell (hMSC) lines were ...

Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City 700000, Vietnam Vietnam National University, Ho Chi Minh City ...

mice in which doxycycline induced frataxin knockout. Western blot analysis of mitochondrial markers revealed significant differences between FRDAkd and KIKO mice. The authors observed that frataxin ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. An evolutionarily conserved micropeptide, ...

New faces, old heads, stars of tomorrow and everyone in between. Sports reporter covering the length and breadth of Western NSW. Got a yarn? Send me an email via dominic.unwin@austcommunitymedia ...

The development of precise and efficient delivery systems is pivotal for advancing CRISPR/Cas9 gene-editing technologies, particularly for therapeutic applications. Engineered metal–organic frameworks ...

Western blotting allows scientists to identify specific proteins in complex biological samples and determine relative abundance of a protein target. Rebecca Roberts is a science writer and ...