Figure 1: Approaches to genetic perturbation: shRNA knockdown, Cas9 ... for knockout or transcriptional activation screening, respectively. Immunohistochemistry and western blotting are two ...

AMSBIO has established a comprehensive portfolio of knock-out cell lines and lysates generated with CRISPR/Cas9 technology ... cell lines and provide the perfect negative control for Western Blots to ...

The rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, and transcriptional regulation. This allows researchers to more ...

CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic models both for fundamental and preclinical research. Despite its apparent simplicity, the outcome of a ...

This will allow quicker and easier gene knock-down experiments, for example, because having on-board Cas9 makes more room in vectors used to deliver the guide RNA to contain multiple instructions for ...

The CRISPR-Cas9 system requires a Cas9 nuclease and a guide RNA, which may consist of either a CRISPR RNA (crRNA) coupled with a trans-activating crRNA (tracrRNA), or a single guide RNA (sgRNA) that ...

The effectiveness of CAR T cell therapy against a variety of cancers, including solid tumors, could be boosted greatly by using CRISPR-Cas9 technology to knock out the gene for CD5, a protein ...

The rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, and transcriptional regulation. This allows researchers to more ...