Northwestern Medicine investigators have developed a new avenue to reprogram compromised immune cells to act against tumors, ...

This study presents an optimized CRISPR-Cas12a system for large-scale genetic screening, revealing critical genes involved in ...

This changed with the development of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 genome editing ... system is the ‘gold standard’ of creating conditional knockout alleles ...

Methods We applied empagliflozin treatment to lupus-prone MRL/lpr mice to explore the renal protective potential of SGLT2 inhibitors. An SGLT2 knockout monoclonal podocyte cell line was generated ...

1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900 USA 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical ...

For the first time, scientists have created a bi-paternal mouse – that is ... To address these limitations, the team used a CRISPR/Cas9-based gene editing technology to target 20 genes ...

CRISPR-Cas9-based genetic screens are a powerful new tool in biology ... Here, we use recently-devised sgRNA design rules to create human and mouse genome-wide libraries, perform positive and negative ...

Using CRISPR-Cas9 gene-editing tools to knock out each of 23,000 genes in old mice, they found more than 300 genes that, when silenced, resulted in restored neural stem cell function. Taking a closer ...

4 Among them, 2 phase 1 clinical trials are active in the cardiac field, with CRISPR/Cas9 knock-out of the transthyretin gene in familial transthyretin amyloidosis (NTLA-2001 [Intellia Therapeutics]) ...

CRISPR/cas9 was used to establish IFNAR1 KO cells. Four sgRNAs were designed according to the ... the rabbits were euthanized and bled to obtain the antiserum. The mouse monoclonal antibodies against ...