KI of an mRFP sequence in spider major ampullate silk using CRISPR-Cas9. Credit: Angewandte Chemie International Edition (2025). DOI: 10.1002/anie.202502068 ...

For a decade, leading academic institutes and their associated companies fought a bruising, headline-grabbing fight over who held patent rights to CRISPR-Cas9, the revolutionary genome editing tool.

Subsequently, DNA repair pathways like non-homologous end joining (NHEJ) or homology-directed repair bring the Cas9 and sgRNA together to ultimately modify the genome. The incorporation of CRISPR ...

The gene editing technique CRISPR/Cas9 has allowed researchers to make precise and impactful changes to an organism's DNA to fix mutations that cause genetic disease. However, the CRISPR/Cas9 ...

Whilst genome editing with CRISPR-Cas9 is effective, it runs the risk of off-target effects and excessive mutations, which can lead to toxicity. To reduce the likelihood of these occurring, ...

CRISPR-Cas9 gene editing technology is widely used to to help study genes of interest and modify disease-associated genes. However, the system is associated with adverse effects, including ...

Researchers are working to reveal potential threats to the efficacy of CRISPR/Cas9 gene editing, even when it appears to be working as planned. A Rice University lab is leading the effort to ...

A new class of spherical nucleic acids has been developed to deliver CRISPR-Cas9 machinery across cell membranes and into the nucleus, without the need for transfection agents. A research group led by ...

Now, scientists at the University of Texas (UT) at Austin have refined the Cas9 protein used in the Nobel Prize-winning CRISPR-Cas9 tool. The new version, dubbed SuperFi-Cas9, was thousands of ...

CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations. Nature Communications , 2022; 13 (1) DOI: 10.1038/s41467-022-28244-5 Cite ...

A comprehensive study headed by researchers at Sanford Burnham Prebys has shown that gene editing—specifically gene knockout (KO)—using CRISPR-Cas9 technology can favor cells with mutated ...