CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease ... a method using a dual stem-loop DNA signal amplifier ...

Guided by their understanding of how IscBs and Cas9s interact with both DNA and their RNA guides, the researchers made additional changes, aiming to optimize both efficiency and specificity.

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In this valuable study, the authors demonstrate that TCF7L2 plays a role in the pathogenesis of cachexia in a mouse model of GI cancer. The results are solid, although future studies will need further ...

With zero-touch recovery, multi-network connectivity, and real-time network intelligence, DNA 140 fuses advanced Next-Generation Firewall (NGFW), intelligent SD-WAN routing, dual 5G failover ...

HIV viral load tests look for RNA, the part of HIV that has the recipe for reproducing itself. They add an enzyme, a kind of protein, to make more copies of the RNA. This makes it easier to ...

First, RNA extraction ... Tris-HCl pH 7.9, 50 mM NaCl, 10 mM MgCl 2, and 100 μg/mL bovine serum albumin; NEB). 50 nM CRISPR-Cas12a ribonucleoprotein, previously assembled at room temperature for 30 ...

A conserved α-helix motif of HDIP1 binds to architectural DNA and nucleosomes and is required for the function of HDIP1 in Arabidopsis. Here, we identified three previously uncharacterized homologous ...