a transformed human embryonic kidney fibroblast cell line that is easily transfected, and suspension Jurkat cells, a human lymphoma/leukemia cell line that is difficult to transfect. shRNA against ...

The technical note describes the optimal electroporation conditions developed for Jurkat cells, a difficult-to-transfect cell line, using the Gene Pulser MXcell electroporation system and Gene Pulser® ...

Jurkat cells were resuspended in the hypoosmolar ... be most suitable in the previous approach (Figure 2). The cells were transfected in triplicate with two Cdc2-specific siRNA and a nonsilencing ...

Researchers at the National University of Singapore (NUS) have developed a scalable, non-viral technology that efficiently ...

Various approaches to transfection are utilized, ranging from electroporation and the use of nanoparticles to viral vector-based methods. The choice depends on both the nature of the project and ...

The flow cytometry and gene sequencing results showed that the cell viability of mesenchymal stem cells and primary T cells post-transfection remained at approximately 83.9% and 84.4% ...

In a new study, researchers have synthesized new complex-hybrid compounds named triptycene-peptide hybrids (TPHs), which successfully induced a kind of PCD known as paraptosis in Jurkat cells - -a ...