The 3D environment allows cells to develop more naturally, as they would in the embryo. In this protocol, we describe a 3D culture method for the generation of inner ear sensory epithelia ...

Depending on the cell's proliferation rate, which varies among different lines, the initial plating density of cells needs to ... Also, we compare the culture protocols for the induction of ...

To conduct research requiring cell culture work and to perform fundamental cell culture protocols, there are several key pieces ... reduce the risk of ice crystal formation which can damage cells and ...

Indeed, Lyle adds, current stem cell culture protocols probably haven’t reached anywhere near the optimal conditions for efficient growth and division. “There is still a lot of area for improvement,” ...

High variability and low consistency are major problems in manual stem cell culture processes ... making it difficult to reproduce experimental protocols and interpret the results obtained.

Neurites begin to extend, reminiscent of dendrites and/or axons Cell recovery: Obtain cells and thaw them quickly at 37 °C. Gently remove cell suspension from tube and add to a petri dish containing ...

This specialized integrated fluidic circuit will enable a variety of cell culture applications, including allowing stem cell researchers to simplify and automate protocols for turning ...

Enriching the stem cell culture with CD177 at the endoderm stage ... disease modelling and drug screening Current beta cell differentiation protocols generate very heterogeneous cell populations ...

During this, the cell culture took up a "rostral ... "Our work shows that the differentiation protocol can develop safe and efficient pluripotent stem cell-based therapies for age-related macular ...