In this experiment, we used both adherent cells (HeLa) and suspension cells (lymphoblastoid cells, LCL) and chose to detect the nucleolar protein PES1 (pescadillo ribosomal biogenesis factor 1, Fig.

The nucleolus is a giant ribosome biogenesis center ... retroelements - I'm anthropomorphizing them - have gone into the ribosomal DNA. It's multicopy, it's conserved, and it's a safe harbor ...

to produce and assemble mature ribosomal proteins (rRNAs) Most ribosome biogenesis occurs in the nucleolus, beginning with the transcription of 47S pre-rRNA from ribosomal DNA loci by the enzyme ...

The schematic depicts various stages of ribosome biogenesis within the nucleolar compartment. The rRNA synthesis, modification, and maturation takes place in the nucleolus following which, they ...

However, throughout its biogenesis over 200 assembly factors are known to sequentially and transiently associate with pre-ribosomal intermediates that mature as they are trafficked from their origin ...

"The nucleolus is perceived as a basic housekeeper: It's responsible for producing ribosomal RNA ... enlarged nucleoli with increased ribosome biogenesis. It is still unclear why a small nucleolus ...

United Kingdom The nucleolus is the most conspicuous domain in the eukaryotic cell nucleus, whose main function is ribosomal RNA (rRNA) synthesis and ribosome biogenesis. However, there is growing ...

After import, it is unclear how these intrinsically unstable and aggregation-prone proteins are targeted to the ribosome assembly site in the nucleolus ... not stably associate with pre-ribosomal ...

This suggests that the disrupted nucleoli may provide a platform for L5- and L11-dependent p53 activation, implying a role for the nucleolus in p53 activation by ribosomal biogenesis stress. These ...