Plasmid vectors pET11a-link-NGFP and pMRBAD ... by digesting 10 μl of each miniprep with the appropriate pair of restriction enzymes and separating the fragments by electrophoresis through ...

For example, when the first lockup for Facebook ... and large shareholders—are prohibited from selling their shares. The restriction is part of a lockup agreement made between the company ...

To study this, we co-transfected FLp53 along with either Δ133p53 or Δ160p53 expressing plasmid and measured FLp53’s binding ... A fraction of the cleared cell lysates was reserved as the input sample; ...

The RNA samples were reverse transcribed into cDNA, and real-time fluorescence-based quantitative PCR experiments were conducted, with each sample being repeated ... digested with Nco1 and Pml1 ...

The Drosophila Gateway™ Vector collection is a set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies. At its core is Invitrogen's Gateway™ ...

and the fluorescence signals were quantified using Living Image 3.0. 2.10 T-cell response by ELISpot The specific IFNγ-secreting T-cell response was measured via an enzyme-linked immunospot (ELISpot).

Department of Applied Chemistry and Biotechnology, Chiba University, Chiba 263-8522, Japan ...