A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

"This is a rare example in biotechnology ... researchers rely on finding a restriction enzyme whose cut site appears only once in the genome of their organism or plasmid -- an often difficult ...

Recombinase-based methods allow scientists to move pieces of DNA from plasmid to plasmid without restriction enzymes, simplifying and expediting the cloning process. It also prevents the aggravation ...

If you decide to use the multiplication module, make sure the restriction enzymes used for multiplication ... tails of PCR primers in Step 3). The plasmid used as vector also can be efficiently ...

The Ti plasmid is removed from the agrobacterium cell, and a restriction enzyme cleaves the T-DNA at a specific restriction site. Subsequently, foreign DNA, which has been cleaved by the same enzyme, ...