Prepare plasmid DNA using standard miniprep protocols ... 10 μl of each miniprep with the appropriate pair of restriction enzymes and separating the fragments by electrophoresis through an ...

DNA is often seen as the blueprint of life—carrying the code to govern the development and traits of an organism—but “there ...

Enzyme mechanisms are the chemical transformations, and the steps within them, generated by enzymatic action on substrates. The mechanism of enzyme catalysis is similar in principle to other types ...

and the fluorescence signals were quantified using Living Image 3.0. 2.10 T-cell response by ELISpot The specific IFNγ-secreting T-cell response was measured via an enzyme-linked immunospot (ELISpot).