Although this technique used DNA polymerase repeatedly, similar to PCR, it employed only a single primer template complex, and exponential amplification was not possible using this technique.

Toyouchi, Tokonami, and Iida write. By eliminating the need for PCR amplification, this method reduces costs, simplifies testing, and accelerates results, making genetic analysis more accessible ...

RPA operates at a constant low temperature (37-45 °C) with only two specific primers, offering a rapid and sensitive alternative to PCR. In this webinar, we will demonstrate the use of RPA reaction as ...