design of flow cytometry panels (including assessment of the myeloid compartment), important considerations for blood sampling during humanized mouse experiments, chimerism calculation methods ...

Strengths: Flow cytometry combined with spleen bulk and single cell transcriptomics ... that differential role of CD47 and TSP1 in extramedullary erythropoiesis in mouse spleen. Furthermore, the ...

Flow cytometry analysis was carried out by using FL1 (488 nm/525 nm, Calcein AM) and FL3 (488 nm/620 nm, PI) channels. 4) The steps for detecting the number of M1/M2 macrophages in the spleen were as ...

Methods To address this, a 4 timepoint longitudinal study was conducted using four different spontaneous SLE mouse models: MRL/lpr, NZB/W, BXSB.Yaa, and TLR7.Tg6. RNA-Seq from whole-blood, spleen, and ...

Because of its speed and ability to scrutinize at the single-cell level, flow cytometry offers the cell biologist the statistical power to rapidly analyze and characterize millions of cells.

Representative flow cytometry histograms of pure RBC or RBC attached with ... In addition, the freshly collected mouse spleen of each group was placed in an embedding box and then the OCT was added to ...

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In addition, mouse spleen was studied to determine at which age erythropoietic ... Bone marrow and spleen cells were stained for flow cytometry as previously described (Mayer et al., 2017). Gating ...