This study provides valuable findings on the role of site-specific DNA methylation changes during spermatogenesis and their contribution to paternal epigenetic inheritance. The study proposes that ...

For example, in March 2023, Illumina Inc., a U.S.-based biotechnology company, introduced Illumina Complete Long Read Technology, which facilitates both long-read and short-read sequencing on a ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. In response to infections, naive CD8 T cells ...

DNA extraction was performed after remodeling, and the samples were processed using the EZ DNA Methylation-Gold Kit (ZYMO, CA ... high-throughput sequencing was performed on the Illumina Hiseq/Miseq ...

The tandem mtZFNs allow efficient packaging into a single AAV, reducing the mtDNA mutation load in cardiac and skeletal muscle tissues in a mouse model. This approach showed significant molecular ...

and the KAPA HyperPrep kit (Roche #KK8504) following manufacturer’s protocols and using Illumina TruSeq dual indexes. Library size was checked by TapeStation 4200 (Agilent) and quality control (QC) ...

In this study, human and mouse ... (Illumina) using a total of 340 ng DNA. DNA was isolated via phenol/chloroform extraction and isopropanol precipitation according to standard protocol. Organoid RNA ...

The cDNA was fragmented using the Nextera XT DNA Library Preparation Kit (Illumina) with the following program: 55°C for 5 min, cooled to 10°C. Adapter ligation was conducted using the Nextera XT ...

Generic but comprehensive pipeline for prokaryotic genome annotation and interrogation with interactive reports and shiny app.