ChVLig encircles the DNA as a C-shaped protein clamp. The NTase domain binds to the broken and intact DNA strands in the major groove flanking the nick and also in the minor groove on the 3’-OH side ...

This first loop sits in the major groove and makes sequence-specific contacts for all six proteins. The number of contacts, and therefore the overall contribution to DNA binding specificity is ...

Compaction of DNA involves charge neutralization in combination with stabilization of inter-helix interactions. The compaction agent binds in either the major or minor groove, in direct contact ...