Therefore, dissociated tumoroids were used for further experiments. We then compared lipofection, electroporation, and lentivirus-based transduction in a difficult-to-transfect model, focusing on ...

Background: Current lipid-lowering drugs rely on the LDL receptor (LDLR) pathway to lower LDL cholesterol. This leaves patients with null mutations in the LDLR, such as those with homozygous familial ...

Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo 113-0033, Japan Department of Biochemistry, Juntendo University Graduate School of Medicine, Tokyo ...

Background: Inhibitors of angiopoietin-like3 (ANGPTL3) reduce low-density lipoprotein (LDL) cholesterol, even in Familial Hypercholesterolemia (FH) patients with a dysfunctional LDL receptor (LDLR).

DUBLIN--(BUSINESS WIRE)--The "Transfection Technologies Market: Global Industry Trends, Share, Size, Growth, Opportunity and Forecast 2023-2028" report has been added to ResearchAndMarkets.com's ...

Transfection using cationic liposomal lipids is termed as lipofection and involves the formation of positively charged lipid aggregates surrounding the negatively charged nucleic acid molecules that ...

Current methods, such as lipofection and viral method, cannot effectively deliver materials in some cell types (e.g., cells with abundant extracellular matrix(ECM). Moreover, physical methods, such as ...

2018; Hsu et al., 2019). In this study, we focused on testing a non-integrative and simple system to mediate efficient gene editing in GS cells, being Cas9:gRNA ribonucleoprotein (RNP) complexes ...

Direct reprogramming based on genetic factors resembles a promising strategy to replace lost cells in degenerative diseases such as Parkinson's disease. For this, we developed a knock‐in mouse line ...