Lentiviral transduction is a popular delivery platform for applications such as CAR-T cell engineering or CRISPR-Cas9 gene editing, as it can transduce both dividing and non-dividing cells and ...

Lentiviral transduction is a popular and powerful gene delivery method for downstream applications such as cell line generation, therapeutic model generation and CAR-T research. However, current ...

Transduction involves using a non-replicating virus, like a modified lentivirus, to bring genetic material into the cell; transfection involves introducing genetic material using a non-viral method.

To improve the efficacy of gene therapy when using anti-sickling beta globin gene transfer, they added cyclosporin (CsH) to increase transduction by inhibiting lentiviral restriction factors.

Lentiviruses have the unique ability amongst retroviruses of being able to infect non-cycling cells. Vectors derived from lentiviruses have provided a huge advancement in technology and seemingly ...

More specific, local administration of lentiviral vectors into the retina or brain resulted in a stable transduction of differentiated cells. RNA interference (RNAi) represents a ...

Autologous HSCs were transduced with CD68-ET3-LV — a lentiviral vector including a new F8 transgene (ET3) with a myeloid-directed CD68 promoter — either without transduction enhancer (group 1 ...

Viral gene delivery is a ubiquitous laboratory technique with a myraid of uses. Understanding the basics of gene delivery workflows, from vector selection and cloning to transduction, is a fundamental ...

Lentiviral transduction of producer cells, followed by expansion of transduced cells, amplified the fraction of producer cells with a single barcode-capsid variant pair. The barcoded eVLP capsid ...

With the fast progress and deeper understanding of CAR-T cell therapy, finding a better solution other than lentivirus transduction is more requested, to overcome the functional limitation on random ...