Greater numbers of circulating stem cells increase the efficacy of in vivo gene therapy techniques, potentially reducing the price tag of lifesaving treatments.

researchers are more frequently selecting lentiviral transduction because (1) it provides long-lasting gene expression, (2) it is compatible with knock-in, knock-out, and knock-down CRISPR ...

Lentiviral transduction is a popular and powerful gene delivery method for downstream applications such as cell line generation, therapeutic model generation and CAR-T research. However, current ...

Casgevy uses the gene-editing technology CRISPR/Cas9 to boost the production of fetal hemoglobin in HSCs. Lyfgenia provides a modified HBB gene, delivered to HSCs using a lentivirus carrier ... the ...

CRISPR-based technologies have broadly enabled genome editing ... To generate dCas9 KRAB plasmids for all candidate dCas9s, we modified a lentiviral expression plasmid (Addgene no. 83925) by removing ...

HeLa cells were co-transfected with Nup358 CRISPR-Cas9 (pCRISPR-CG01 ... inducible shRNA HEK293T cell lines for shControl and shNup358 were generated using lentivirus transduction. To generate ...

We conducted genome-wide CRISPR screens in isogenic RPE1 cells expressing ... according to the manufacturer’s protocols. For all lentiviral transduction for in vitro (cultured cells) experiments, ...

ISG-Targeted CRISPR Screen Identifies RNF213 as the Primary Determinant of IFN-γ-Mediated Growth Restriction in Multiple Human Cell Types. Cloning and Lentiviral Transduction of the sgRNA Libraries. A ...

As a more recently discovered technology, CRISPR ... a Lentiviral vector, researchers transduced the Cas9 complex into the cells to eliminate the need to generate a cell line specifically for Cas9 ...

Cytidine deamination that is guided by clustered regularly interspaced short palindromic repeats (CRISPR ... cytometry after base editing and lentiviral transduction with high-level disruption ...