The authors confirm that telomeres in telomerase-positive cells terminate with 5'-ATC in a Pot1-dependent manner, and demonstrate that this principle holds true in telomerase-negative ALT cells as ...

ClickFix is a social engineering trick that hackers have been using more and more since early 2024 to spread malware. It fools you into running malicious commands on your own computer, and the ...

Selection was first performed under kanamycin resistance conditions to integrate the plasmid into ... the knockout vector, and tRNA deletion strains with anticodon UUU were expected to be obtained ...

PWWP2B interacts with MRE11 and participates in homologous recombination via promoting DNA ... (E) The siPWWP2B‐resistant PWWP2B WT expression vector was transfected into HeLa cells after ...

Adenovirus-based vaccines are prepared by inserting a transgene cassette into the adenoviral backbone through direct cloning or homologous recombination. The transgene cassette expresses the ...

Our vector series, pGTag (plasmids for ... arm-directed targeted integration of a GFP reporter into safe harbor loci in porcine and human cells increased 10-fold in comparison to homologous ...

AAVHSCs can be designed to gene edit using the homologous recombination pathway ... designed to insert a functional copy of the gene into a vector flanked by long stretches of DNA sequences ...

Multiple loss-of-function alterations in genes that are involved in DNA repair, including homologous recombination repair, are associated with response to poly ...

The prevalence of homologous recombination DNA damage repair (HR-DDR) deficiencies among all tumor lineages is not well characterized. Therapy directed toward homologous recombination DDR deficiency ...

Thus, the approach to identify biomarkers will need to take temporal tumor evolution into consideration ... and massively parallel assessment of molecular phenotypes of homologous recombination ...

Here, we introduce the CRISPR/Cas9 as a site-specific gene knock-in instrument that promotes the homologs recombination of a linearized ... another PRV virus Bartha was also subcloned into a BAC with ...