To test this approach, we inserted a green fluorescent protein (GFP) nanobody (GFPnb) into the VP1 protein of AAV-DJ inside the capsid and replaced the AAV payload plasmid with a GFP expression ...

20 µL of this mixture was transferred to a 0.5 ml protein LoBind tube and mixed with 640 pmol of SPYtag-GFP nanobody and incubated at 4 °C for 10 h. The beads were washed twice and resuspended with 25 ...

Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States Institute for Medical Engineering and Science, Massachusetts ...

Here, we have developed and used a novel, nanobody-based toolset, the GrabFP system, to interfere with the localization of GFP-tagged proteins along the apical-basal axis in the larval wing imaginal ...