The authors provide a detailed step-by-step description of MagIC-Cryo-EM procedure including nucleosome isolation, preparation of GFP nanobody attached magnetic beads, optimization of the spacer ...

20 µL of this mixture was transferred to a 0.5 ml protein LoBind tube and mixed with 640 pmol of SPYtag-GFP nanobody and incubated at 4 °C for 10 h. The beads were washed twice and resuspended with 25 ...

This review provides an in-depth analysis of the latest applications of nanobody-based strategies in targeting various components of the tumor microenvironment for both therapeutic and diagnostic ...

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Organic Chemistry, College of Chemistry and ...

Department of Chemistry, University of California, Berkeley, California 94720, United States Department of Chemistry, University of California, Berkeley, California 94720, United States Department of ...

A common strategy to identify and morphologically analyze neural cells is to genetically express freely diffusible cytosolic or membrane labels, such as green or red fluorescent protein (GFP or RFP).

For convenience, the data folder can be organized as follows. MonteCarloSampling contains the code for de novo nanobody design that integrates AiPPA with thermodynamic Monte Carlo sampling, as ...