EGFP was fluorescently quantified on a DeNovix QFX fluorometer using the BioVision GFP Quantitation Kit. A 200 μL total assay volume was prepared in 0.5 mL, thin-wall PCR tubes. To make 1 μg/μL ...

In the FPP assay, adapted from biochemical protease protection assays, GFP fusion proteins are used as noninvasive tools to obtain details of protein topology and localization within living cells ...