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Viral-mediated Oct4 overexpression induces direct conversion of wildtype mammalian Müller glia to bipolar neurons, and this process is synergistically enhanced by loss of Notch signaling.
Only Stx1–pHluorin localized at the PM was surface-labelled with anti-GFP and Alexa 647-tagged secondary antibodies and visualized by STORM imaging. mEOS2–Sec22b or Sec22b–Dendra2 were ...